The present study aimed to evaluate antiviral and anticancer potentials of some Egyptian snake, scorpion venoms and L-amino acid Oxidase as well. Regarding venoms cytotoxicity and physiological changes to cancer and lab animals respectively data revealed that all test venoms have a prominent cytotoxic effect to CACO-2 and MCF -7 cell lines compared to normal cell control (MRC-5). Cytotoxic effect of test venoms to cancer cells extremely exceeds that to normal cells, as the difference reach up to 80 fold in case of LAO enzyme and up to 63 fold in case of Leiurus scorpion venom. Apoptotic gene regulation was assessed using semi quantitative RT-PCR. Results revealed that there was a marked up regulation for pro-apoptotic genes (P53 & BAX) post treatment with Cerastes cerastes, Naja haja, Leiurus venoms and LAO enzyme. Also down regulatory effect for anti-apoptotic gene (Bcl-2) was recorded post cellular treatment with Cerastes cerastes, Leiurus venom and LAO enzyme. A marked physiological changes were detected post treatment of induced mass and ascetic fluid using different test venoms especially for Cerastes cerastes and Naja nigricollis venoms where a marked leucopenia in addition to lympho and monocytosis as well as changes in liver enzymes post venom treatment accompanied with marked decrease in size of tumor mass, ascetic fluid volume, EAC count in ascetic fluid and differences in body weight approaching to the normal values. Antiviral activity evaluation recorded a marked reduction in HSV-II titer by 30% in Vero treated cells by different snake venoms and 40% for Leiurus venom while treatment of cells with LAO enzyme reduced the virus titer by 15%. Regarding VSV antiviral activity of test venoms, data indicated a reduction in the virus titer by 10% in case of LAO, 30% in case of Naja nigricollis treatment and 15% reduction in other test venoms. Both snake and scorpion venoms even LAO enzyme tend to record a more viricidal potentials compared with antiviral one. VSV was completely inactivated within 6hr post treatment by all test venoms as well LAO enzyme while reduction rate of VSV was 75% reduction post treatment with Cerastes cerastes venom. HSV-II depleted titer was 50% for Cerastes cerastes, Naja nigricollis and 65% for other test venoms 6 hr post treatment.
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